anti araf (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
anti araf
Anti Araf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+araf/A-Raf+Antibody/bio_rxiv__64898__2026__03__25__713956-419-58-59
Average 94 stars, based on 121 article reviews
Anti Araf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+araf/A-Raf+Antibody/bio_rxiv__64898__2026__03__25__713956-419-58-59
Average 94 stars, based on 121 article reviews
anti araf - by Bioz Stars,
2026-09
94/100 stars
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other:Article Title: The stability of R-spine defines RAF inhibitor resistance: A comprehensive analysis of oncogenic BRAF mutants with in-frame insertion of αC-β4 loop Article Snippet: Antibodies used in this study include anti–phospho-ERK1/2 (no. 4370), anti–phospho-MEK1/2 (no. 9154), anti-MEK1/2 (no. 9124), anti-ARAF (no. 4432), and Staining:Article Title: New level of regulation of MITF activity by direct interaction with RAF proteins Article Snippet: HEK293T cells were seeded at 3.105 cells per well in 6-wells slides (Millicell, Millipore) precoated with poly-L-lysine (Sigma) and transfected with pcDNA3/myc-MITF and pcDNA3/HA-ARAF, pcDNA3/HA-BRAF, pcDNA3/HA-CRAF plasmids or pcDNA3 empty vector as previously described. .. After 48 hours, cells were fixed in 4 % paraformaldehyde, blocked (0.1 % Triton X-100, 10 % goat serum in PBS) and stained overnight at 4 °C with anti-myc antibody (Santa Cruz) and Article Title: MITF activity is regulated by a direct interaction with RAF proteins in melanoma cells Article Snippet: HEK293T cells were seeded at 3.10 5 cells per well in 6-wells slides (Millicell, Millipore) precoated with poly-L-lysine (Sigma) and transfected with pcdna3-myc-MITF and pcdna3-RAF plasmids (HA-ARAF, HA-BRAF or HA-CRAF) or empty vector as previously described. .. After 48 hours, cells were fixed in 4% paraformaldehyde, blocked (0.1% Triton X-100, 10% goat serum in PBS) and stained overnight at 4 °C with anti-myc antibody (Santa Cruz) and Article Title: MITF activity is regulated by a direct interaction with RAF proteins in melanoma cells. Article Snippet: HEK293T cells were seeded at 3.105 cells per well in 6-wells slides (Millicell, Millipore) precoated with poly-L-lysine (Sigma) and transfected with pcdna3-myc-MITF and pcdna3-RAF plasmids (HA-ARAF, HA-BRAF or HA-CRAF) or empty vector as previously described. .. After 48 hours, cells were fixed in 4% paraformaldehyde, blocked (0.1% Triton X-100, 10% goat serum in PBS) and stained overnight at 4 °C with anti-myc antibody (Santa Cruz) and Blocking Assay:Article Title: The HSP90–CDC37 Chaperone System Orchestrates RAF1 Kinase Activation Through a Pre-Dimerization Mechanism Article Snippet: Twenty micrograms of whole-cell lysate or one-tenth of the eluted samples after the double affinity purifications was separated by SDS-PAGE using NuPAGETM 4–12% Bis-Tris Midi Gels (ThermoFisher Scientific) and transferred to nitrocellulose blotting membranes (Cytiva Life Sciences). .. Following blocking with 5% BSA in TBS-T for 1 hour at RT, the membranes were incubated with the following primary antibodies: anti-HSP90 (Santa Cruz, sc-13119; 1:1,000), anti-RAF1 (BD, 610151; 1:1,000), anti-CDC37 (Santa Cruz, sc-13129; 1:1,000), anti-pan-Ras (Calbiochem, OP40; 1:1,000), anti-V5-tag (ThermoFisher Scientific, R960-25; 1:1,000), anti-StrepTagII (ThermoFisher Scientific, MAS-37747; 1:500), anti-Vinculin (Sigma, V9131; 1:5,000), anti-BRAF (Santa Cruz, sc-5284; 1:1,000), Incubation:Article Title: The HSP90–CDC37 Chaperone System Orchestrates RAF1 Kinase Activation Through a Pre-Dimerization Mechanism Article Snippet: Twenty micrograms of whole-cell lysate or one-tenth of the eluted samples after the double affinity purifications was separated by SDS-PAGE using NuPAGETM 4–12% Bis-Tris Midi Gels (ThermoFisher Scientific) and transferred to nitrocellulose blotting membranes (Cytiva Life Sciences). .. Following blocking with 5% BSA in TBS-T for 1 hour at RT, the membranes were incubated with the following primary antibodies: anti-HSP90 (Santa Cruz, sc-13119; 1:1,000), anti-RAF1 (BD, 610151; 1:1,000), anti-CDC37 (Santa Cruz, sc-13129; 1:1,000), anti-pan-Ras (Calbiochem, OP40; 1:1,000), anti-V5-tag (ThermoFisher Scientific, R960-25; 1:1,000), anti-StrepTagII (ThermoFisher Scientific, MAS-37747; 1:500), anti-Vinculin (Sigma, V9131; 1:5,000), anti-BRAF (Santa Cruz, sc-5284; 1:1,000), |